A multi-endpoint in vivo larval zebrafish (Danio rerio) model for the assessment of integrated cardiovascular function


Despite effective in vitro preclinical strategies to identify cardiovascular (CV) liabilities, there remains a need for early functional assessment prior to complex in vivo mammalian models. The larval zebrafish (Danio rerio, Zf) has been suggested for this role: previous data suggest that cardiac electrophysiology and vascular ultrastructure are comparable with mammals, and also indicate responsiveness of individual Zf CV system endpoints to some functional modulators. Little information is, however, available regarding integrated functional CV responses to drug treatment. Consequently, we developed a novel larval Zf model capable of simultaneous quantification of chronotropic, inotropic and arrhythmic effects, alongside measures of blood flow [...]

Heart Beat and blood Flow

Fig. 1. Annotated composite screen shots of the (A) MicroZebraLab™ program used to quantify ABR and VBR, and (B) ZebraBlood™ program used to quantify blood flow and vessel diameter. In both cases tracking areas are defined to allow the detection of ‘motion’. The scale for vessel diameter is provided using a haemocytometer grid. Other annotations signify anatomical reference points.

Thomas Parkera, Paul-Antoine Libourel, Malcolm J. Hetheridge, Robert I. Cumminga, Thomas P. Sutcliffea, Alexander C. Goonesinghe, Jonathan S. Balla, Stewart F. Owen, Yann Chomis, Matthew J. Winter

Volume 69, Issue 1, January–February 2014, Pages 30–38

This publication was performed using the MicroZebraLab