Neurobehavioral effects of two metabolites of BDE-47 (6-OH-BDE-47 and 6-MeO-BDE-47) on zebrafish larvae


The neurobehavioral toxicity of 6-OH/MeO-BDE47 were evaluated with zebrafish larvae.

6-OH-BDE47 performed inhibiting effects on routine and average turns.

Different from 6-OH-BDE-47, 6-MeO-BDE47 mainly promoted responsive turns.

6-MeO-BDE-47 performed more adverse effects on social activity.


Two metabolites, OH-BDEs and MeO-BDEs, of polybrominated diphenyl ethers (PBDEs) were ubiquitously detected in animal tissues and environmental samples, drawing a widely public concern to their toxicity. The comparison of toxicity between PBDEs and their metabolites has been a focus in recent years, however, comparisons seldom involve neurobehavioral toxicity of PBDEs metabolites in published works. In this study, zebrafish larvae were exposed to 6-OH-BDE-47 and 6-MeO-BDE-47 and their neurobehavioral traits (including locomotion, path angle, and social activity) were recorded using the instrument Zebrabox; meanwhile, light illumination was used as stimuli in the test duration. The results showed larvae were more active in dark periods than light periods, and preferred turning right (+) to left (−). Effects of the two metabolites varied in different behavioral indicators. They induced different effects on path angle but did not reverse the left-right asymmetry. 6-OH-BDE-47 did not induce the effects on larval locomotion and social activity, but mainly decreased average and routine turn numbers; 6-MeO-BDE-47 promoted larvae responsive turns but inhibited social activity. This study offered new experimental means to the neurobehavioral toxicity of various PBDE metabolites. Further studies may focus on the toxic mechanisms of specific neurobehavioral traits.


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