ZebraBox for embryos or larvae

Long-term observation is a possibility by maintaining environmental conditions constant. The Temperature Control Unit deliverable with the ZebraBox system allows to continuous control over experimental conditions. Temperature can be controlled from 20°C to 30°C. The system is equipped with an UV-C water clarifier to avoid algae and bacterial build-up.

ZebraBox can be equipped with an additional module to create reproducible and standardized sound and vibration stimuli, the vibration module.

Research using a shock paradigm can easily be standardized with ZebraBox. Thanks to the shocker, enjoy the reproducibility of standardizing your work combined with the automation provided by ZebraBox.

This package includes adapted filters for the camera and light sources to create a system for the analysis of PhotoMotor Response in zebrafish embryos. Analysis can happen as of 36 hpf, and continue as needed.

It now features the ability to record very fast behaviors such as startle reflex and PPI analysis, revolutionizing the way you observe larvae in micro-well plates. You are now able to check behaviors that happen in few milliseconds !

Camera at 1000 FPS and electronic modules. Option to perform tests such as Zebrafish prepulse inhibition and sensorimotor gating / PPI.

The Zebrafish Aggression Box – ZAB is a brand new solution developed by ViewPoint in collaboration with Dr. William Norton team (University of Leicester) to monitor aggression in one-month-old juvenile zebrafish.

Our videotracking technology has been combined with a compact and practical cubical (50 cm each side) where up to 4 zebrafish can be placed and analyzed simultaneously. The system is lit with infrared for a perfect detection of the zebrafish.

The software detects fish aggression based upon the reaction of the fish facing its own image towards a mirror. It can automatically quantify zebrafish aggression and locomotion.

 Targeted applications :

→ Automatic quantification can detect alterations to aggression levels caused by drug treatment or mutation.

→ This solution is relevant to screen for drugs that decrease zebrafish aggression.

 Advantages :

→ Validated software by comparison to manual quantification of behavior.

→ Scalable system : ability to monitor an unlimited number of fish, allowing you to speed up your test. Indeed, you can work with as many cabinets as you want, which are connected to the same system.

 Outputs :

 → Aggression Index : aggressive behavior pattern/intensity.

"The development of the Zebrafish Aggression Box has been supported by the European Commission Framework Programme (FP7/2007-2013) under grant agreement n°602805"

Experimental device supply allowing to test active avoidance on larvae or adult zebrafish, with visual, sound and electric stimuli.

Dimensions of the tank for adult :

Length = 30 cm, Width = 18 cm, Height = 15 cm

• Each tank will have 2 compartments of 15 cm length separated by a removable wall equipped with a mouth of a shape and dimensions to define before manufacturing.

• The 2 compartments will be equipped with stainless steel plates placed at the bottom and at the surface enabling the electric shocks to be delivered.

Dimensions of the tank for larvae :

Length = 129 mm, Width = 86 mm, Height = 28 mm

• The tank is divided into 8 compartments (2 rows of 4), for up to 8 larvae at the same time. One compartment is sized 34*29 mm with two areas of 14 mm wide.

Visual and electric stimuli will be controlled by the ZebraLab software.

The complete test device will be composed of :

• A tank as described above,

• An individual rack resistant to the shock generator,

• A voltage-controllable generator for the electric stimuli,

• An independent visual stimuli system for each compartment.

The optomotor response of zebrafish is a reflex to stabilize the body position in a moving environment Optomotor response measurements are done using a moving grating as stimulus that is presented by our sytstem. A fullscreen squarewave grating, which moves left- or rightwards. The user can adjust speed, contrast, number of stripes.

ZOMR  is a component of ZebraLab and ZebraBox and allows the automated observation and tracking of  zebrafish larvea  visual behavior in a freely moving environment. In a few steps, start monitoring the visual-motor response inside Petry dish, 6 wells Microplate, or up to 10 corridors /alleys.

Optogenetic is a breakthrough technic in neurosciences research that is quickly adopted all around the world.

Researchers naturally asked ViewPoint how to implement such a tool for zebrafish larvae ; leveraging the advantage of the transparent nature of embryos and high-throughput capabilities of this model.

We have been working closely with researchers' teams across the world to bring a straight forward approach to high-throughput optogenetic screening. Thanks to expert teams of researchers at IGF Montpellier, Icrea Barcelona and Berkeley California, we are proud to bring you the new ZebraBox Revo add-on for Optogenetic & Opto pharmacology.

Thanks to the versatile nature of ZebraBox Revo, our community of users can easily adapt this technology to their current system and make it part of their pipeline.

Stay tuned for more upcoming information on Optogenetic with the ZebraBox. Or contact us to order your solution now.

Discover the ZebraBox apparatus and its many applications.

Please browse the following publication

Light&sound proof Zebra box allows experiment in very tight contrable environement.
Designed with a specialized lab working on auditory and visual field, this collaboration pushed a step
forward our zebrabox to experiment in the best efficients conditions for behavioral asssays.
• Easy to install from a factory regular ZebraBox,
• No lens or camera adjustment needed.
• Versatile equipement, fully adapted to all our add ons ( TCU, Optogenetic, Vibration module)
• Allow dimlight experiment in complet darkness.
• Reducing and attenuating interference sound by 40 DB gain
• Dimension on the bench: Around 75cm X 77cm X 30 cm
• Weight : 22 Kilos

Aquatic Light/Dark Preference test for pharmacological, drug screening application and anxiety, stress
/preference tests on larval zebrafish in microplates compatible with the ZebraBox with the following
specifications:
 light-dark grid available for 48, 24, 12, 6 well microplate1 formats
 Each well has one white and one black side
 Grid is placed underneath the well plate
 Infrared lighting translucent material for dark condition tracking
 Customizable size on request
1Only compatible with Grinder microplateAquatic Light/Dark Preference test for pharmacological, drug screening application and anxiety, stress
/preference tests on larval zebrafish in microplates compatible with the ZebraBox with the following
specifications:
 light-dark grid available for 48, 24, 12, 6 well microplate1 formats
 Each well has one white and one black side
 Grid is placed underneath the well plate
 Infrared lighting translucent material for dark condition tracking
 Customizable size on request
1Only compatible with Grinder microplate

Extension of the ZebraLab system for Rotation and Histograms including:
 High-Throughput screening detection of up to four arenas simultaneously per camera
 Animal Rotation measurement:
- Body rotation count
- Circling behavior
- Clock wise/Counter clockwise rotation count
 Path Angle Histograms:
- Path Angle (changes of direction of trajectories along the subject’s path)
- Lateral body shifting / Lateral Swerving tendencies
- Behavior classification with eight different class of angles
- User defined class angle

Extension of the ZebraLab system for Social contact and interaction including:
High-Throughput screening detection up to four arenas simultaneously per camera
Social behavior measurement for up to two animals housed in a single arena
- Distance between two animals
- Animal to Animal contacts detection (contact count)
- Time spent in which two animals are within each other’s proximity
- Fusion; sexual behavior copulation

Extension of the ZebraLab system for Group locomotion tracking of different subjects in the same
region, including:
 Group behavior analysis
- Inter-individual distance (Mean distance from each subject to all others)
- Nearest neighbor distance (Mean Distance between the subject and its nearest neighbor)
- Polarization (Magnitude of the mean vector of all subjects)
- Speed Momentary speed for each fish (mean)